Fig 2: Expression levels of UCN3 and HSPs during the differentiation of 3T3-L1 preadipocytes and their treatment with a macrophage culture medium (MaCM). mRNA expression levels of UCN3, HSP90, GRP78, CHOP, HSP72, and HSP60 were measured by quantitative real-time PCR in 3T3-L1 preadipocytes (d0) and adipocytes differentiated for 8 days (d8) with and without MaCM. GAPDH was used as an internal control for normalization and data are presented as fold changes in differentiated adipocytes compared with preadipocytes from independent experiments (n = 4). *p < 0.05.

Fig 3: Relative changes (%) in GRP78 levels at 24 h after cardiopulmonary bypass (CPB; H24-CPB) in patients with or without organ failure. The results show that GRP78 levels remain below baseline in patients with organ failure while they return to baseline in patients without organ failure (with no difference in proteinemia between the two groups), suggesting a less intense unfolded protein response in patients with organ failure. Dosages were performed by Enzyme linked immunosorbent assay (ELISA). Values are presented as median with interquartile range. **p < 0.01 between groups. GRP78, 78 kDa Glucose-Regulated Protein.

Fig 4: Plasma levels of GRP78 (A), VCAM-1 (B), Syndecan-1 (C) and IL-6 (D) before (Pre-CPB), 2 (H2-CPB) and 24 (H24-CPB) hours after cardiopulmonary bypass (CPB). The results show a post-operative increase in IL-6, Syndecan-1 and VCAM-1 and a transient decrease (at H2-CPB only) in GRP78. Dosages were performed by Enzyme linked immunosorbent assay (ELISA). Values are presented as median with interquartile range. ***p < 0.001 in comparison with Pre-CPB level. GRP78, 78 kDa Glucose-Regulated Protein; IL-6, Interleukin 6; VCAM-1, Vascular Cell Adhesion Protein 1.

Fig 5: Relative levels of studied protein 2 (H2-CPB) and 24 (H24-CPB) hours after cardiopulmonary bypass (CPB). The results show a post-operative relative increase in Syndecan-1 and VCAM-1 and a transient decrease (2 h after CPB only) in GRP78. Dosages were performed by Enzyme linked immunosorbent assay (ELISA). Values are presented as median with interquartile range. ***p < 0.001 in comparison with Pre-CPB level. GRP78, 78 kDa Glucose-Regulated Protein; VCAM-1, Vascular Cell Adhesion Protein 1.