Fig 1: Senolytic treatment improves systemic metabolism but does not restore vasopressin-related defects in aged mice.a. Experimental design: Mice were divided into D+Q and Sham gavage experimental groups. b. Weight changes over time of old (n = 7-8) and young (n = 3-4). c. Representative images of D+Q and Sham-gavage mice. d. RT-qPCR of p16, p21 and Tnfa expression in gWAT biopsies after 9 weeks of treatment. e-i. Indirect calorimetry assessment of D+Q or Sham-treated mice measuring (e) VO2, (f) RER, (g) X+Y locomotor activity, (h) water intake, and (i) food intake. j. Osmolality derived from plasma, k-l. Core T measurements at 22°C and 28°C in the light and dark phases and (k) under 4°C challenge (l). m-q. Quantification of morphological features of AVP+ neurons in each region: (m) AVP signal intensity per ROI, (n) ROI size, (o) AVP cell bodies per ROI, (p) AVP cell body size, and (q) AVP signal intensity per cell. Data are means ± sem. One-way ANOVA or Student’s t-test (bar graphs), two-way ANOVA (line-graphs) were used. p-values: ns = p > 0.05; *** = p < 0.005; **** = p < 0.0005.
Fig 2: Single-nuclei RNA sequencing of the aging anterior hypothalamus.a. Schematic illustrating workflow of microdissection and age groups pooled into snRNA-seq (n=4). b. UMAP of all nuclei from both experimental groups, processed into 29 clusters. c. UMAP of 5 major cell types identified in dataset. d. UMAP of subset neural clusters, identifying 25 subclusters. e. Cell proportions across each cell type split by age. f. g. Volcano plot of differentially expressed genes in aging vs. young nuclei pools. h-i. UMAP of inhibitory and excitatory neurons with respective clusters. j. Violin plot of Avp expression across Avp-rich clusters from excitatory (e13, e15, e8) and inhibitory (i15, i20, i21, i23) subclusters, with increased Avp expression observed in e15. k. Dot plot of key gene markers in Avp-rich clusters including Sim1 (marker of PVN and SON neurons) and Rgs16 (labels SCN neurons) shows that high e15 expression of Avp coincides with Sim1 expression.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Mouse Copeptin ELISA Kit (Colorimetric)