Description
The Porcine Progesterone (Pg) ELISA quantitates Pg in serum and plasma.
Principle of the method
The Porcine Pg solid phase competitive ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. Porcine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. A substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The enzyme substrate reaction is terminated by the addition of stop solution and the color turns yellow. The intensity of this signal, measured spectrophotometrically at a wavelength of 450±2 nm, is directly proportional to the concentration of target present in the original specimen.
Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization