Fig 1: Oocyte quality declines in association with abnormal PLTP and cholesterol metabolism of follicular fluid in PCOS patients. (A) Volcano plot of DEPs between HP and LP group. Up- and downregulated DEPs with p < 0.01 and fold change > 2 are highlighted in light red and light blue, respectively. (B) Proteomic sequencing results for PLTP expression in five PCOS patients with high rate of high-quality D3 embryos (HP) group and five PCOS patients with low rate of high-quality D3 embryos (LP) group. (C) Proteomic sequencing results for HYOU1 expression in HP group and LP group. (D) The co-expression network of DEPs in HP group and LP group. (E) ELISA assay analysis of PLTP protein levels in the follicular fluid between HP and LP groups. (F) ELISA assay analysis of HYOU1 protein levels in the follicular fluid between HP and LP groups. (G-I) The expression levels of triglycerides (TG) (G), total cholesterol (CHOL) (H), and high-density lipoprotein (HDL) (I) in follicular fluid between HP and LP groups. Statistical significance is denoted by *p < 0.05, **p < 0.01, ***p < 0.001.
Fig 2: Validation of hub protein concentration levels in follicular fluid associated with oocyte quality by ELISA. (A-F) The differential concentration levels of HYOU1 (A), PLTP (B), NCAM1 (C), MCSF1 (D), IGFBP1 (E), and VNN1 (F) were further validated using follicular fluid samples collected from 38 controls and 42 PCOS patients. The concentrations of selected proteins in follicular fluid samples were measured by commercial ELISA kit. Statistical significance is denoted by *p < 0.05, ***p < 0.001, ****p < 0.0001.
Supplier Page from Abcam for Human PLTP ELISA Kit