Fig 1: TNC is a prognostic factor that correlates with the albumin concentration, neutrophil-to-lymphocyte ratio and carbohydrate antigen 19–9 (CA19-9) concentration. (a) Serum TNC levels of pancreatic cancer patients and healthy controls (control, n = 64; stage 0-IV, n = 140; Mann‒Whitney U test). (b) Serum TNC levels and trend analysis of pancreatic cancer patients and healthy controls staged according to the UICC 8th edition. *, p < 0.05. (control, n = 64; stage 0-II, n = 50; stage III-IV, n = 90; p = 0.003, Jonckheere-Terpstra trend test). (c) Patients with stage 0-IV disease were analyzed (n = 140). Pearson’s correlation coefficients for TNC and albumin concentrations, the neutrophil-to-lymphocyte ratio, body mass index, CEA level, and CA19-9 level were calculated. (d) Survival curves of 77 patients with metastatic pancreatic cancer in the TNC-low and TNC-high groups according to the Kaplan‒Meier method (TNC-low group: n = 39; TNC-high group: n = 38; log rank test). *, p < 0.05.
Fig 2: RNA sequencing of tumors from high-fat diet (HFD)-fed mice and normal diet (ND)-fed mice revealed serum tenascin C as a candidate serum marker for obesity-related development of murine orthotopic pancreatic tumors. Mice fed an ND or an HFD from 5 weeks of age to 15 weeks of age were orthotopically injected with murine-derived mPKC1 pancreatic cancer cells. (a) Scheme of the protocol for orthotopic injection of ND- and HFD-fed mice (ND: n = 10, HFD: n = 9). Body weight (BW) at 15 weeks of age, tumor weight 3 weeks after orthotopic injection, and serum nonesterified fatty acid (NEFA) levels 3 weeks after orthotopic injection of ND- and HFD-fed mice. (b) Survival curve of ND- and HFD-fed mice after orthotopic injection (log rank test). (c) RNA sequencing of tumors from ND- and HFD-fed mice was performed. Among twenty-nine genes with FPKM values that were 2 times greater in HFD-fed tumors than in ND-fed tumors and FPKM > 10 in HFD-fed tumors, 11 genes with FPKM values below 10 in adipose tissues are shown. Three genes (IL1f9, Cxcl3, TNC) encode secreted proteins according to the Human Protein Atlas (www.proteinatlas.org). (d) Real-time PCR of tumors from ND- and HFD-fed mice. (e) Serum TNC levels in ND- and HFD-fed mice. Mann‒Whitney U test; bar only, median; *, p < 0.05.
Supplier Page from Abcam for Human Tenascin C ELISA Kit