Fig 2: Production of (A) SOD, (B) glutathione, (C) MDA, and (D) NFκB in A549 cells after treatment with 2,4D-NPs or raw 2,4D for 24 h. The results are expressed as mean ± SE of three replicates. *Significantly different at p < 0.05 (Tukey post hoc test). ****Significantly different at p < 0.001 (Tukey post hoc test). n.s. non-statistically significant. 2,4D-NPs refers to 2,4D-loaded nanoparticles. Note that the concentrations shown on the x-axis represent the actual 2,4D content within the nanoparticle formulation. All treatment concentrations for both the 2,4D-NPs and raw 2,4D groups were standardized to contain an equivalent amount of 2,4D.

Fig 3: Correlation analysis between miR-941 and factors related to the Nrf2/Keap1 signaling pathway and the NIHSS score is retained here. Spearman correlation analysis between NIHSS and A miR-941, B Nrf2 mRNA, C Keap1 mRNA, D HO-1, E NQO-1, F SOD, and G GSH-Px

Fig 4: The combination treatment of DOX and CUR against H69AR cells after 24 h treatment. (a) iron concentrations, (b) MDA, (c) GSH, (d) SOD, (e) TrxR. Cells were treated with DOX and/or CUR at the following concentrations: DOX IC10 = 9.2 µM, IC20 = 20.8 µM, IC30 = 35.6 µM; CUR IC10 = 8.5 µM, IC20 = 19.2 µM, IC30 = 32.9 µM. Data are expressed as mean ± SD (n = 3 independent experiments). * Significantly different at p < 0.05 (Tukey post hoc test). ** Significantly different at p < 0.005 (Tukey post hoc test). **** Significantly different at p < 0.001 (Tukey post hoc test). n.s. non-statistically significant.

Fig 5: Predictive efficacy of miR-941 and factors related to the Nrf2/Keap1 signaling pathway in the prognosis diagnosis of elderly ischemic stroke patients. ROC curves of A miR-941, B Nrf2 mRNA, C Keap1 mRNA, D HO-1, E NQO-1, F SOD, and G GSH-Px in the effectiveness of pathological diagnosis in elderly stroke patients