Fig 1: TSG-6 mediates UCMSC suppression of DC maturation, function, and CCR6+ Th17 differentiation. (A) Flow cytometry was used to detect the expression of surface mature markers CD14, CD1a, and CD83 in each group, along with their quantitative statistical graphs (n = 6). (B) Flow cytometry was employed to detect the expression of surface functional markers CD40, CD86, and HLA-DR in each group, together with their quantitative statistical graphs (n = 6). (C) Flow cytometry was utilized to detect the proportion of IL17+CCR6+ Th17 cells in each group of mDC after co-culture with mixed lymphocytes for 3 days, as well as their quantitative statistical graphs (n = 6). (D, E) Immunofluorescence was used to detect representative images and quantitative statistical graphs of the number of peripheral and central DCs in the corneas of each group of mice (n = 6). (F) Flow cytometry was applied to detect the number and quantitative statistical graphs of CD45+CD11c+DC in the corneas, conjunctiva, and draining lymph nodes of each group of mice (n = 6). (G, H) Flow cytometry was used to detect the number and quantitative statistical graphs of CD11c+CD86+ DC and CD11c+MHC-II+ DC in the corneas, conjunctiva, and draining lymph nodes of each group of mice (n = 6). Data are presented as the mean ± SD. Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001.