Description
BioVendor Human soluble uPAR (urokinase-type plasminogen activator receptor, uPAR: suPAR, soluble plasminogen activator urokinase receptor, soluble urokinase-type plasminogen activator receptor, PLAUR, sPLAUR) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human suPAR in serum, plasma, and cell culture supernatants. The assay employs an antibody specific for human suPAR coated on a 96-well plate. Standards and samples are pipetted into the wells and suPAR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human suPAR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of suPAR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm