Description
The BioVendor Human FGF-7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF-7 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human FGF-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FGF-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm