Description
The BioVendor Human IGFBP-4 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP-4 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human IGFBP-4 coated on a 96well plate. Standards and samples are pipetted into the wells and IGFBP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGFBP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm