Description
This detection kit is applicable for the quantitative detection of residual DNase I content in test samples by double-antibody sandwich method. The standard and test samples are added to reaction wells on microplate pre-coated with DNase I-specific monoclonal antibodies for incubation. The present DNase I quantitatively binds to the antibodies in the microplate, while unbound substances are removed after plate washing. Add Anti-DNase I mAb (detection antibody) and H + L secondary antibodies successively (enzyme conjugate) to form an antibody - antigen - antibody - secondary antibody complex. The protein content in the test sample can be indicated by the color development degree of TMB. Please read the instructions for use carefully and check the components of the kit before use