Description
This kit is a sandwich enzyme immunoassay for the quantitative determination of rat NT-proBNP in serum and plasma samples. In a first step, STD/CTRL/Sample (Standard, Control, Sample) and detection antibody (biotinylated polyclonal anti-rat NT-proBNP antibody, AB) are pipetted into the respective wells of the microtiter strips, which are pre-coated with a polyclonal anti-rat NT-proBNP antibody. Rat NT-proBNP present in the sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. After a first wash step, which removes non-specifically unbound material, the conjugate (Streptavidin-HRPO) is added and reacts with the detection antibody. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed color change of the substrate is directly proportional to the amount of rat NT-proBNP present in the sample. This color change is detectable with a standard microtiter plate ELISA reader. The concentration of rat NT-proBNP in the sample is determined directly from the dose response curve. The kit utilizes recombinant rat NT-proBNP as a calibrator