Description
Designed for simple and rapid isolation of high quality PCR-ready genomic DNA from blood
The isolated DNA is free of RNA and contaminants that may inhibit PCR. The isolated DNA is of high integrity and high molecular weight, and PCR-results show excellent reproducibility in combination with high level of sensitivity. The process of DNA isolation relies upon cell lysis to release DNA and adsorption of DNA to the surface of Dynabeads. This is followed by magnetic separation of intact DNA/Dynabeads complex and subsequent washing to remove any residual contaminants and potential PCR inhibitors from isolated DNA. Finally, complex is resuspended for direct use in downstream PCR-reactions. Alternatively, DNA may be eluted from Dynabeads following short incubation at 65°C. Multiple samples may be handled in parallel and isolation process can be automated.
- Cost-effective, simple and efficient single-tube procedure, completed in only 15 minutes
- Supports 100 isolations from 100µL of blood per sample
- Sufficient for at least 100 PCR amplifications
- Protocol can be modified to allow isolation from 500µL blood samples, providing enough template DNA for 1,000 PCR amplifications
- Isolation of PCR-ready genomic DNA directly from blood
- < 100µL Blood Samples (Small-Scale), 100µL - 1mL Blood Samples ( Medium-Scale)
Automated Genomic DNA Purification, Automated Nucleic Acid Purification, DNA and RNA Purification and Analysis, DNA Extraction, Genomic DNA Purification, gDNA from Blood (Medium-Scale, 100 µl-1mL), gDNA from Blood (Small-Scale, < 100 µl)