Description
This Mouse Uteroglobulin-Related Protein 2 ELISA Kit is intended for quantitative detection of mouse SCGB3A1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse SCGB3A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SCGB3A1 has been precoated onto 96-well plates. Standards (Expression system for standard: E Coli, Immunogen sequence: F22-G104) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB3A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SCGB3A1 amount of sample captured in plate.
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. This gene encodes a small secreted protein that may function in the inhibition of cell growth. Expression of the related gene in humans is downregulated via DNA methylation in breast cancers and other tumor types. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene