Description
This Mouse Semaphorin 6C ELISA Kit is intended for quantitative detection of mouse SEMA6C in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This mouse SEMA6C ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SEMA6C has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A26-P605) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEMA6C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SEMA6C amount of sample captured in plate.
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Semaphorin-6C is a protein that in humans is encoded by the SEMA6C gene. This gene is mapped to 3; 3 F2.1. This gene product is a member of the semaphoring family of proteins. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration