Description
Human Ephrin A1 CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Ephrin A1 in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Human EFNA1 in samples. No significant cross-reactivity or interference between Human EFNA1 and analogues was observed. Our Ephrin A1 CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EFNA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EFNA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EFNA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EFNA1. You can calculate the concentration of Human EFNA1 in the samples by comparing the RLU value of the samples to the standard curve