Description
This Human Interleukin-38 (IL-38) ELISA Kit is intended for Quantitative Detection of human IL-38 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human IL-38 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-38 has been precoated onto 96-well plates. Standards (Expression system for standard: E Coli, Immunogen sequence: M1-W152) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-38 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-38 amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Interleukin-1 family member 10 is a protein that in humans is encoded by the IL1F10 gene. The protein encoded by thi s gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses