Description
Human Tamm-Horsfall Glycoprotein CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Tamm-Horsfall Glycoprotein in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Human THP in samples. No significant cross-reactivity or interference between Human THP and analogues was observed. Our Tamm-Horsfall Glycoprotein CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human THP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human THP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human THP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human THP. You can calculate the concentration of Human THP in the samples by comparing the RLU value of the samples to the standard curve