Description
Rat Heat Shock Protein CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Heat Shock Protein in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Rat HSP47 in samples. No significant cross-reactivity or interference between Rat HSP47 and analogues was observed. Our Heat Shock Protein CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP47. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP47 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP47, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSP47. You can calculate the concentration of Rat HSP47 in the samples by comparing the RLU value of the samples to the standard curve