Description
This Monkey Keratinocyte Growth Factor (FGF7) High-Sensitivity ELISA Kit is intended is a high-sensitivity sandwich ELISA kit for quantitative detection of monkey primate FGF7/KGF. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests.
This Monkey FGF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF7 has been precoated onto 96-well plates. Standards (E Coli, C32-T194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey FGF7 amount of sample captured in plate.
The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of monkey primate and monkey primate homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis