Description
Human Delta Like 1 Homolog CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Delta Like 1 Homolog in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Human DLK-1 in samples. No significant cross-reactivity or interference between Human DLK-1 and analogues was observed. Our Delta Like 1 Homolog CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DLK-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DLK-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DLK-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DLK-1. You can calculate the concentration of Human DLK-1 in the samples by comparing the RLU value of the samples to the standard curve