Description
The Human Rubella IgM ELISA Kit is intended for use in the detection of IgM antibody to the rubella virus. Strip well format. Reagents for up to 96 tests.
Purified Rubella antigen is coated on the surface of microwells. Diluted patient serum is added to the wells, and the Rubella IgM- specific antibody, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, antibody to human IgM conjugated with horseradish peroxidase (HRP) is added and incubated at 37 degree C for 30 minutes. Unbound conjugate is removed by a subsequent washing step. A solution of TMB Reagent is then added to the microwells. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls