Description
The Zearalenone quantitative test is based on the principle of the enzyme-linked immunosorbent as-say. An antibody binding protein is coated on the surface of a microtiter plate. Zearalenone containing samples or standards, a zearalenone-peroxidase conjugate and an antibody directed against zeara-lenone are given into the wells of the microtiter plate. The conjugate competes with the zearalenone of samples/standards for the limited number of antibody sites. Simultaneously the anti-zearalenone anti-body is bound to the antibody-binding protein coated on the microtiter plate. After 10 minutes incuba-tion at room temperature the wells are washed with diluted washing solution to remove unbound mate-rial. A substrate solution is added and incubated for 10 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yel-low. The yellow colour is measured photometrically at 450 nm. The concentration of zearalenone is indirectly proportional to the colour intensity of the test sample