Description
This Human Amyloid-Like Protein 1 ELISA Kit is intended for quantitative detection of human APLP1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human APLP1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APLP1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S39-E580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APLP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APLP1 amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated monoclonal antibody from goat. Expression system for standard: This gene encodes a member of the highly conserved amyloid precursor protein gene family. It is mapped to 19q13.12. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described