Mouse Plasmin-Antiplasmin Complex CLIA Kit from MyBioSource.com

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Mouse Plasmin-Antiplasmin Complex CLIA Kit

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Description

Mouse Plasmin-Antiplasmin Complex CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Plasmin-Antiplasmin Complex in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Mouse PAP in samples. No significant cross-reactivity or interference between Mouse PAP and analogues was observed. Our Plasmin-Antiplasmin Complex CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PAP. You can calculate the concentration of Mouse PAP in the samples by comparing the RLU value of the samples to the standard curve