Description
Mouse NGB ELISA Kit is a highly sensitive sandwich enzyme-linked immunoassay (ELISA) for measuring NGB in serum, plasma and other biological fluids. Reagents for up to 96 tests.
This ELISA Kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this Kit has been pre-coated with an antibody specific to Mouse NGB. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Mouse NGB. You can calculate the concentration of Mouse NGB in the samples by comparing the OD of the samples to the standard curve