Description
This Mouse Apolipoprotein J (Clusterin) ELISA Kit is intended for quantitative detection of Mouse Clusterin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse Clusterin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Clusterin has been precoated onto 96-well plates. Standards (NSO, D22-R226(beta) & S227-E448(alpha)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Clusterin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Clusterin amount of sample captured in plate