Description
The improved guanidine isothiocyanate/phenol one-step method (TRIzol method) lyses cells and inactivates RNase, and then the total RNA is selectively adsorbed on the silicon matrix membrane in the spin column in a highly isolated salt state. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica membrane with RNase-free H2O