Description
This Rat Plexin A1 ELISA Kit is intended for quantitative detection of rat Plexin A1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This rat Plexin A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Plexin A1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S46-S1255) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Plexin A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Plexin-A1 is a protein that in humans is encoded by the PLXNA1 gene. This gene is mapped to 4q34. Plexin A1 has been shown to interact with AKT1