Description
This Human T-Lymphocyte Surface Antigen Ly 9 ELISA Kit is intended for quantitative detection of human LY9 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human LY9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LY9 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: K48-K454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LY9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 17q21.31. LY9 has also recently been designated CD229. LY9 belongs to the SLAM family of immunomodulatory receptors and interacts with the adaptor molecule SAP