Description
Human Semaphorin 3A CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Semaphorin 3A in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Human SEMA3A in samples. No significant cross-reactivity or interference between Human SEMA3A and analogues was observed. Our Semaphorin 3A CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA3A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA3A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SEMA3A. You can calculate the concentration of Human SEMA3A in the samples by comparing the RLU value of the samples to the standard curve