Description
This Rat Semaphorin 3F ELISA Kit is intended for quantitative detection of rat SEMA3F in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This rat SEMA3F ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SEMA3F has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A19-T785) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEMA3F is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. It is mapped to 8q32. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G, chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K, a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family