Description
Human Tight Junction Protein 1 CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Tight Junction Protein 1 in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Human TJP1 in samples. No significant cross-reactivity or interference between Human TJP1 and analogues was observed. Our Tight Junction Protein 1 CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TJP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TJP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TJP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TJP1. You can calculate the concentration of Human TJP1 in the samples by comparing the RLU value of the samples to the standard curve