Description
This Human Antithrombin III Rapid ELISA Kit is intended is a fast ELISA kit, this assay takes less than 1.5 hours to detect Human Antithrombin Iii/SERPINC1 with <10pg/ml sensitivity. format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Antithrombin Iii/SERPINC1 tissue specificity: Found in plasma. Strip well format. Reagents for up to 96 tests.
This human Serpin C1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin C1 has been precoated onto 96-well plates. Standards (NSO, H33-K464) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Serpin C1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Serpin C1 amount of sample captured in plate.
The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%)