Description
This Monkey Survivin (BIRC5) High-Sensitivity ELISA Kit is intended is a high-sensitivity sandwich ELISA kit for quantitative detection of monkey primate Survivin. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests.
This Monkey Survivin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Survivin has been precoated onto 96-well plates. Standards (E Coli, M1-D142) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Survivin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey Survivin amount of sample captured in plate.
The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: Survivin (also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common monkey primate cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant monkey primate Survivin with the molecular mass of 34 KDa