Description
Multi-Species MEK ELISA Kit is a highly sensitive competitive enzyme-linked immunoassay (ELISA) for measuring MEK in serum, plasma and other biological fluids. Reagents for up to 96 tests.
This ELISA Kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this Kit has been pre-coated with MEK. During the reaction, MEK in samples or Standard competes with a fixed amount of MEK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MEK. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The concentration of MEK in the samples is then determined by comparing the OD of the samples to the standard curve