Description
This Mouse Growth Differentiation Factor 15 ELISA Kit is intended for quantitative detection of mouse GDF-15 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for GDF-15 has been precoated onto 96-well plates. Standards (NSO, S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GDF-15 amount of sample captured in plate.
The capture antibody is a polyclonal antibody from sheep, the detection antibody is a biotinylated polyclonal antibody from sheep. Expression system for standard: GDF-15 (Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation