Description
The Multi-Species Glucose Fluorescent Detection Kit is designed to quantitatively measure glucose in a variety of samples. Strip well format. Reagents for up to 96 tests.
The kit is designed to quantitatively measure glucose in a variety of samples. Please read the complete kit insert before performing this assay. A beta-D-glucose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Substrate and horseradish peroxidase and the reaction initiated by addition of glucose oxidase. The reaction is incubated at room tem-perature for 30 minutes. The glucose oxidase reacts with glucose to produce hydrogen peroxide which, in the presence of HRP, converts the substrate into a fluorescent product. The fluorescent product is read at 590 nm with excitation at 570 nm. Increasing levels of glucose cause a linear increase in fluorescence