Description
The Multi-Species Hemoglobin Colorimetric Detection Kit is designed to quantitatively measure all forms of hemoglobin present in blood and RBCs, or plasma and serum. Strip well format. Reagents for up to 96 tests.
The kit is designed to quantitatively measure all forms of hemoglobin present in blood and RBCs, or plasma and serum. Please read the complete kit insert before performing this assay. A human hemoglobin standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate and the ready-to-use Hemoglobin Detection Reagent is added to each well. For whole blood or RBC samples 10 uL of samples and standards are used in the Regular format, for serum and plasma samples 100 uL are used in the High Sensitivity format. Results are calculated as g/dL for whole blood and RBCs, andmg/ml for serum and plasma. The plate is incubated for 30 minutes at room temperature. The plate is read at 560-580 nm to detect the intensity of the color generated. The concentration of the hemoglobin in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers