Description
Rat Creatine Kinase MB Isoenzyme CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Creatine Kinase MB Isoenzyme in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Rat CK-MB in samples. No significant cross-reactivity or interference between Rat CK-MB and analogues was observed. Our Creatine Kinase MB Isoenzyme CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CK-MB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CK-MB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CK-MB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CK-MB. You can calculate the concentration of Rat CK-MB in the samples by comparing the RLU value of the samples to the standard curve