Description
Human Secreted Frizzled Related Protein 2 CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Secreted Frizzled Related Protein 2 in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Human SFRP2 in samples. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed. Our Secreted Frizzled Related Protein 2 CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SFRP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SFRP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SFRP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SFRP2. You can calculate the concentration of Human SFRP2 in the samples by comparing the RLU value of the samples to the standard curve