Description
This Mouse Insulin-like Growth Factor Binding Protein 1 ELISA Kit is intended for quantitative detection of mouse IGFBP-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IGFBP-1 has been precoated onto 96-well plates. Standards (NSO, A26-N272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP-1 amount of sample captured in plate.
The capture antibody is a polyclonal antibody from goat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KDa