Description
Intended Uses: The Triiodothyronine (T3) ELISA Kit is intended for the quantitative measurement of Triiodothyronine (T3) in human serum. For research use only -
Principle of the Assay: The T3 is a solid phases competitive ELISA. The samples, assay buffer and T3 enzyme conjugate are added to the wells coated with anti-T3 monoclonal antibody. T3 in the serum competes with a T3 enzyme conjugate for binding sites. Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3