Description
This Human Roundabout Homolog 1 (ROBO1) ELISA Kit is intended for quantitative detection of human ROBO1 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.
This human ROBO1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ROBO1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: Q26-P897) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ROBO1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. This gene is mapped to 3p12.3. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene