Description
This Human Metalloproteinase Inhibitor 2 Rapid ELISA Kit is intended is a fast ELISA kit, this assay takes less than 1.5 hours to detect Human TIMP2 with <2pg/ml sensitivity. format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma (heparin, EDTA) and saliva. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. TIMP2 tissue specificity: Strip well format. Reagents for up to 96 tests.
This human TIMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-2 has been precoated onto 96-well plates. Standards (CHO, C27-P220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-2 amount of sample captured in plate.
The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid