Description
Rat Growth Hormone CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Growth Hormone in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Rat GH in samples. No significant cross-reactivity or interference between Rat GH and analogues was observed. Our Growth Hormone CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GH. You can calculate the concentration of Rat GH in the samples by comparing the RLU value of the samples to the standard curve