Description
Creative Biogene Leukemia BCR-ABL Fusion Gene Typing real time RT-PCR kit is used for the detection of Leukemia BCR-ABL Fusion Gene(Major-BCR, minor-BCR, and micro(µ)-BCR) in leukocyte by using real time PCR systems. The Kit contains a specific ready-to-use system for the detection of the Leukemia BCR-ABL Fusion Gene using RT-PCR (Reverse Transcription Polymerase Chain Reaction) in the real-time PCR system. The master contains reagents for the specific amplification of M-BCR, m-BCR and m-BCR. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT), during which the Leukemia BCR-ABL Fusion Gene (M-BCR, m-BCR, µ-BCR) is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by means of PCR (polymerase chain reaction). Fluorescence is emitted and measured by the real time systems optical unit during the PCR