Description
Creative Biogene Coxsackie virus real time PCR kit is used for the detection of coxsackie virus in samples like nasal and pharyngeal secretions, sputum, provoked sputum, stool, C.S.F, serum, etc. The kit contains a specific ready-to-use system for the detection of the Coxsackie Virus A24 using RT-PCR (Reverse Transcription Polymerase Chain Reaction) in the real-time PCR system. The master contains reagents for the specific amplification of the Coxsackie Virus A24 RNA. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT), during which the Coxsackie Virus A24 RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by means of PCR (polymerase chain reaction). Fluorescence is emitted and measured by the real time systems' optical unit during the PCR