Description
Extracellular vesicles such as exosome have attracted attention as a messenger of cellto-cell communication and a biomarker of diseases since they include proteins, mRNAs, microRNAs, and DNAs on their surface or inside and they are stably present in body fluids such as blood, urine, saliva, spinal fluid, and breast milk after being secreted from cells.
This is an enzyme immunoassay kit that is utilizable in the qualitative and quantitative analyses of extracellular vesicles in cell culture supernatant and body fluid samples. The microplate in the kit is pre-coated with protein that specifically binds with phosphatidylserine (PS) on the surface of extracellular vesicles. After extracellular vesicles are captured by the plate, biotinylated antibody for the surface marker protein of extracellular vesicles is used as a primary detection. By using HRP-conjugated streptavidin in the kit as a secondary detection, the extracellular vesicles which have any surface marker proteins can be detected with high sensitivity.
The kit contains biotin-conjugated anti-human CD63 mouse monoclonal antibody as a control detection antibody to detect human CD63-positive extracellular vesicles. This kit can be used for relative quantitation of extracellular vesicles in cell culture supernatant and body fluid samples using purified extracellular vesicles with an interested surface marker protein as a reference standard