Reference sample volume: 10~30uL for rat plasma, the optimum volume is 20uL. The enzyme curve treated with regression curve is relatively proportional in this range. If take sample too many or too less, there will not be obvious difference when taking statistics treatment after experiment.

Reference sample volume: 20~50 μL for 2% mouse brain homogenate, the optimum volume is 35~40 μL. The enzyme curve treated with regression curve is relatively proportional in this range. If take sample too many or too less, there will not be obvious difference when taking statistics treatment after experiment.